| 货号 | 2105S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 使用方法 | WB(1:1000) IHC-P (1:50) |
| 供应商 | CST |
| 背景 | The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Western blot analysis of extracts from NIH/3T3 cells, using Phospho-Src (Tyr527) Antibody (A,B) or v-Src antibody (C,D). The phospho-specificity of the antibody was confirmed by treating the membrane with calf intestinal alkaline phosphatase (CIP) (B,D) after Western transfer. | |
使用Phospho-Src (Tyr527) Antibody兔多抗 (A,B) 和 v-Src antibody兔多抗 (C,D)，对NIH/3T3细胞进行western blot检测。该抗体的磷酸化特异性通过牛小肠磷酸酶（CIP）(B,D)处理转印膜后证实。 |
