| 货号 | 13121S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-SQSTM1/p62 (Thr269/Ser272) Antibody recognizes endogenous levels of SQSTM1 protein only when phosphorylated at Thr269 and Ser272. This antibody may react with either dually or singly phosphorylated SQSTM1/p62. A background band is detected at 75 kDa in some cell lines. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress and autophagy (1-4). It was first identified as a protein that binds to the SH2 domain of p56Lck (5) and independently found to interact with PKCζ (6,7). SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome (8). Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway (9). Protein aggregates formed by SQSTM1 can be degraded by the autophagosome (4,10,11). SQSTM1 binds autophagosomal membrane protein LC3/Atg8, bringing SQSTM1-containing protein aggregates to the autophagosome (12). Lysosomal degradation of autophagosomes leads to a decrease in SQSTM1 levels during autophagy; conversely, autophagy inhibitors stabilize SQSTM1 levels. Studies have demonstrated a link between SQSTM1 and oxidative stress. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress (3). Thus, accumulation of SQSTM1 can lead to an increase in NRF2 activity. |
| 存放说明 | -20C |
| 计算分子量 | 62 |
Immunoprecipitation of Phospho-SQSTM1/p62 (Thr269/Ser272) from ACHN cells using Normal Rabbit IgG #2729 (lane 2) or Phospho-SQSTM1/p62 (Thr262/Ser272) Antibody (lane 3). Lane 1 is 10% input. Western blot was performed using Phospho-SQSTM1/p62 (Thr262/Ser272) Antibody. Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb. 对ACHN细胞中Phospho-SQSTM1/p62 (Thr269/Ser272)进行免疫共沉淀，使用的抗体是Normal Rabbit IgG #2729（泳道2）或Phospho-SQSTM1/p62 (Thr262/Ser272) Antibody（泳道3）。Lane 1代表 10% input。利用Phospho-SQSTM1/p62 (Thr262/Ser272) Antibody进行Western blot检测。Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677作为二抗以避免与IgG重链发生交叉反应。 | |
Western blot analysis of extracts from chloroquine-treated HeLa cells (50 μM, overnight; +) and untreated (-) MCF7 cells, untreated (-) or treated with λ phosphatase and calf intestinal phosphatase (CIP) (+), using Phospho-SQSTM1/p62 (Thr269/Ser272) Antibody (upper) and SQSTM1/p62 (D5E2) Rabbit mAb #8025 (lower). Western blot 分析氯喹处理的HeLa细胞（50uM，过夜;+）和未处理（-）的MCF7细胞提取物，未处理(-)或用λ 磷酸酶和小牛小肠磷酸酶(CIP)处理(+)，使用的抗体是Phospho-SQSTM1/p62 (Thr269/Ser272) Antibody (上图) 和 SQSTM1/p62 (D5E2) Rabbit mAb 兔单抗#8025 (下图)。 | |
Western blot analysis of extracts from A549 cells, untreated (-) or treated with Roscovitine #9885 (20 μM, overnight; +), using Phospho-SQSTM1/p62 (Thr269/Ser272) Antibody (upper), SQSTM1/p62 (D5E2) Rabbit mAb #8025 (middle) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Western blot分析A549细胞的细胞提取物，未处理（-）或Roscovitine #9885处理 (20 μM，过夜;+)，使用的抗体是Phospho-SQSTM1/p62 (Thr269/Ser272) Antibody（上图）、SQSTM1/p62 (D5E2) Rabbit mAb 兔单抗#8025（中图）或β-Actin (D6A8) Rabbit mAb 兔单抗#8457 (下图)。 |
