| 货号 | 4029S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Structural maintenance of chromosomes 1 (SMC1) protein is a chromosomal protein member of the cohesin complex that enables sister chromatid cohesion and plays a role in DNA repair (1,2). ATM/NBS1-dependent phosphorylation of SMC1 occurs at Ser957 and Ser966 in response to ionizing radiation (IR) as part of the intra-S-phase DNA damage checkpoint (3). SMC1 phosphorylation is ATM-independent in cells subjected to other forms of DNA damage, including UV light and hydroxyurea treatment (4). While phosphorylation of SMC1 is required for activation of the IR-induced intra-S-phase checkpoint, the precise mechanism is not well understood and may involve a conformational change that affects SMC1-SMC3 interaction (3).染色体结构维持蛋白1(SMC1)是黏连蛋白复合体的一个染色体蛋白成员，该复合体促进姐妹染色单体结合并在DNA修复中起着重要作用(1,2)。作为S期内部DNA损伤检验点的一部分，ATM/NBS1-依赖的SMC1磷酸化发生在第957位和966位丝氨酸以应答离子辐射(IR) (3)。在经历其他形式的DNA损伤包括紫外线和羟基脲处理的细胞中，SMC1的磷酸化是ATM-非依赖的(4)。尽管SMC1的磷酸化是IR诱导的内S期检验点激活所必需的，但精确的机制尚未阐明且有可能涉及影响SMC1-SMC3相互作用的构象变化(3)。 |
| 存放说明 | -20C |
| 计算分子量 | 145 |
Western blot analysis of extracts from 293 cells, untreated or UV-treated (100 J/m2 followed by 4 hour recovery) using Phospho-SMC1 (Ser360) Antibody (upper) or SMC1 Antibody #4802 (lower). Antibody phospho-specificity was determined by treating cell extracts with λ phosphatase.Western blot 方法检测293细胞提取物，未处理或者紫外处理(100 J/m2 ，随后恢复4 hour)，使用的抗体为Phospho-SMC1 (Ser360) Antibody (上) 或SMC1 Antibody #4802 (下)。抗体的磷酸化特异性通过使用λ 磷酸酶处理细胞来确定。 | |
Confocal immunfluorescent analysis of HeLa cells, untreated (left) and UV-treated (right), using Phospho-SMC1 (Ser360) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).激光共聚焦荧光法检测HeLa细胞提取物，左图未处理，右图为紫外处理，使用的抗体为 Phospho-SMC1 (Ser360) Antibody (绿色)。肌动蛋白纤维使用DY-554 鬼笔环肽标记(红色)。 |
