| 货号 | 9553S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-Smad1 (Ser206) Antibody detects endogenous levels of Smad1 only when phosphorylated at Ser206. No cross reactivity was detected with other famly members. |
| 使用方法 | WB(1:1000) IP (1:25) |
| 供应商 | CST |
| 背景 | Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the coactivating Smad4 and translocate to the nucleus, where they stimulate transcription of target genes (5). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Western blot analysis of ACHN and HeLa cells, untreated or UV-treated, using Phospho-Smad1 (Ser206) Antibody (upper), or total Smad1 Antibody #9743 (lower). 使用Phospho-Smad1 (Ser206) 抗体（上），或total Smad1抗体#9743 （下）对未处理或经过UV处理的ACHN和HeLa细胞进行western blot分析。 |
