| 货号 | 14770S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | SH2 domain-containing leukocyte protein of 76 kDa (SLP-76) is a hematopoietic adapter protein that is important in multiple biochemical signaling pathways and necessary for T cell development and activation (1). ZAP-70 phosphorylates SLP-76 and LAT as a result of TCR ligation. SLP-76 has amino-terminal tyrosine residues followed by a proline rich domain and a carboxy-terminal SH2 domain. Phosphorylation of Tyr113 and Tyr128 result in recruitment of the GEF Vav and the adapter protein Nck (2). TCR ligation also leads to phosphorylation of Tyr145, which mediates an association between SLP-76 and Itk, which is accomplished in part via the proline rich domain of SLP-76 and the SH3 domain of ITK (3). Furthermore, the proline rich domain of SLP-76 binds to the SH3 domains of Grb2-like adapter Gads (3-4). In resting cells, SLP-76 is predominantly in the cytosol. Upon TCR ligation, SLP-76 translocates to the plasma membrane and promotes the assembly of a multi-protein signaling complex that includes Vav, Nck, Itk and PLCg1 (1). The expression of SLP-76 is tightly regulated: the protein is detected at very early stages of thymocyte development, increases as thymocyte maturation progresses, and is reduced as cells mature to CD4+ CD8+ double-positive thymocytes (5). |
| 存放说明 | -20C |
| 计算分子量 | 76 |
Western blot analysis of extracts from Jurkat cells, untreated (-) or H2O2-treated (11 mM, 1 min; +), using Phospho-SLP-76 (Tyr145) Antibody (upper) and SLP-76 Antibody #4958 (lower). | |
Immunoprecipitation of phospho-SLP-76 (Tyr145) from Jurkat cell extracts treated with H2O2 (11 mM, 1 min) using Normal Rabbit IgG #2729 (lane 2) or Phospho-SLP-76 (Tyr145) Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-SLP-76 (Tyr145) Antibody. |
