Phospho-Shc (Tyr239/240) Antibody
货号：
2434S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 2434S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Shc possesses SH2 and PTB domains and serves as a scaffold protein in signaling for a variety of receptor tyrosine kinases. Shc exists in p46, p52 and p66 isoforms, which are produced by using alternative translation initiation sites or a differentially spliced message (1-3). In response to extracellular signals, the SH2 and PTB domains of Shc interact with the activated receptors, leading to phosphorylation of Shc on three different tyrosine residues: Tyr239, Tyr240 and Tyr317 (4-6). GRB2/Sos binds to Shc phosphorylated at these sites, activating the Ras/Raf/MAPK pathway (4). Both Shc expression and its tyrosine phosphorylation play an essential and nonredundant role in thymic T cell development (7). |
| 存放说明 | -20C |
| 计算分子量 | 50, 55, 70 |
参考图片Western blot analysis of extracts from HepG2 cells, untreated or EGF-treated (100 ng/ml) 18 hours of serum-starvation, and Jurkat cells, untreated or treated with anti-CD3 antibody (1 µg/ml for 10 minutes), using Phospho-Shc (Tyr239/240) Antibody (upper) or Shc antibody #2432 (lower).Western blot方法检测细胞提取物：未经处理的和EGF (100 ng/ml)处理的18h无血清培养HepG2细胞，未处理和抗CD3抗体(1 µg/ml 10 min)处理的Jurkat细胞 。使用的抗体是Phospho-Shc (Tyr239/240) Antibody (上图)和Shc antibody #2432(下图). |
