| 货号 | 2215S |
| 反应种属 | Human/Mouse/Rat/Monkey/Zebrafish |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-S6 Ribosomal Protein (Ser240/244) Antibody detects endogenous levels of ribosomal protein S6 only when phosphorylated at serines 240 and 244. This antibody does not detect S6 ribosomal protein phosphorylated at other sites. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 32 |
Western blot analysis of extracts from 293 cells, untreated or treated with 20% FBS for the indicated time, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody #2211 (upper) and Phospho-S6 Ribosomal Protein (Ser240/244) Antibody #2215 (lower). 使用Phospho-S6 Ribosomal Protein (Ser235/236) Antibody #2211 (上图)和Phospho-S6 Ribosomal Protein (Ser240/244) Antibody #2215 (下图)，进行Western blot以分析293细胞中磷酸化S6核糖体蛋白(Ser235/236)和磷酸化S6核糖体蛋白(Ser240/244)的水平，细胞分为对照组和20% FBS处理组(如图示时间指示)。 |
