Phospho-ROS1 (Tyr2274) Antibody
货号：
3078S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 3078S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-Ros (Tyr2274) Antibody detects endogenous levels of Ros only when phosphorylated at tyrosine 2274. This antibody may cross-react with other unknown proteins. |
| 使用方法 | WB(1:1000)
IP (1:50) |
性能| 供应商 | CST |
| 背景 | The c-ros proto-oncogene encodes a receptor tyrosine kinase similar to insulin receptor proteins and expressed in specific epithelial cells (1). During mouse development the Ros receptor plays an important role in the differentiation of epididymal epithelium (2). Overexpression or mutation of Ros may lead to oncogenic development. Furthermore, stable transfection with a chimeric receptor containing the Ros transmembrane and cytoplasmic domains leads to transformation of NIH/3T3 fibroblasts (3). A genetic rearrangement that produces a fusion protein containing Ros and FIG (fused in glioblastoma) results in glioblastoma formation (4). The FIG-Ros fusion abnormally activates the SHP-2 phosphatase and PI3K/Akt/mTOR pathways in tumor cells (5). Additional Ros mutations are associated with NSCLC cancer cells indicating that Ros may be a candidate for tumor targeted therapy (6). |
| 存放说明 | -20C |
| 计算分子量 | 258, 110, 50-80 |
参考图片Western blot analysis of HCC78 cell lysate untreated (upper), or treated with calf intestinal phosphatase (CIP) (middle and lower), using Phospho-Ros (Tyr2274) Antibody (upper and middle), or Ros control antibody (lower). HCC78 cells express a SLC3482-Ros fusion protein which contains an activated Ros kinase fragment. (Rikova, K et al (2007) Cell 131:1190-1203)用Phospho-Ros (Tyr2274) Antibody（上，中）或Ros control antibody（下）对未经处理的（上）或抗体孵育前用intestinal phosphatase (CIP)处理的（中，下）HCC78细胞的裂解液进行免疫印迹检测。HCC78 细胞表达一个SLC3482-Ros 融合蛋白，该蛋白包含一个激活的Ros激酶片段（Rikova, K et al (2007) Cell 131:1190-1203) |
