Phospho-Rev-erbα (Ser55/59) Antibody
货号：
2129S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 2129S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Reverse orientation c-erbA gene α (Rev-erbα, EAR-1, or NR1D1) is a widely expressed member of the orphan nuclear receptor family of proteins (1). Rev-erbα is highly expressed in adipose tissue, skeletal muscle, brain and liver, and regulates cellular proliferation and differentiation. Expression increases during differentiation in adipocytes and ectopic expression of Rev-erbα potentiates the adipocyte differentiation of 3T3-L1 cells (2). In addition, expression oscillates with circadian rhythm in liver cells and Rev-erbα regulates expression of BMAL1, ApoA-I and ApoC-III, all key regulators of circadian rhythm (3,4,5,6,7). Phosphorylation of Rev-erbα Ser55 and Ser59 by GSK3β appears to stabilize Rev-erbα protein levels and is important for synchronizing and maintaining the circadian clock (8). Rev-erbα also regulates inflammation by targeting the NF-κB responsive genes IL-6 and COX-2 (9). Rev-erbα lacks the activation function 2 domain required for ligand-dependent activation of transcription by other members of the nuclear receptor family; thus it behaves as a constitutive repressor protein, recruiting the nuclear receptor co-repressor (N-CoR)/HDAC3 complex to target genes to repress transcription (10).反转定向c-erbA基因α（Rev-erbα, EAR-1或 NR1D1）是一种广泛表达的孤儿核受体蛋白家族成员（1）。Rev-erbα在脂肪组织、骨骼肌、脑和肝中高表达，并调节细胞的增殖和分化。在脂肪细胞分化过程中表达增加，REV-erbα异位表达会增强3T3-L1细胞向脂肪细胞分化（2）。此外，REV-erbα在肝细胞内按昼夜节律振荡表达，并调控BMAL1、ApoA-I和ApoC-III，所有关键调节器都是昼夜节律表达（3,4,5,6,7）。由GSK3β磷酸化REV-erbα的丝氨酸（55和59位），表现出稳定的REV-erbα蛋白质水平，在生物钟的同步和维持中很重要（8）。同样，REV-erbα针对NF-κB应答基因IL-6和COX-2调节炎症（9）。 REV-erbα缺乏激活功能2结构域，这对核受体其他家族成员的配体依赖型转录激活是需要的；因此它作为阻遏蛋白起作用，招募核受体辅阻遏物（N-COR）/ HDAC3复合物的靶基因，抑制转录（10）。 |
| 存放说明 | -20C |
| 计算分子量 | 80 |
参考图片Western blot analysis of extracts from 293 cells, either untransfected or transfected with DYKDDDDK-tagged Rev-erbα expression constructs, using Phospho-Rev-erbα (Ser55/Ser59) Antibody (upper) or DYKDDDDK Tag Antibody (Binds to same epitope as Sigmas Anti-FLAG® M2 Antibody) #2368 (lower). The phospho-specificity of the antibody was verified by transfecting cells with a mutant DYKDDDDK-Rev-erbα (Ser55/59Ala) expression construct and by incubating 293/Rev-erbα (WT) extracts with (+) or without (-) λ phosphatase.Western blot方法检测293细胞提取物，细胞不转染或用DYKDDDDK-标记的表达Rev-erbα的构建体转染，使用的抗体为Phospho-Rev-erbα (Ser55/Ser59) Antibody (上图)或DYKDDDDK Tag Antibody (同Sigmas Anti-FLAG® M2 Antibody一样，结合同一抗原表位) #2368 (下图). 抗体的磷酸化特异性由转染的细胞进行验证，细胞用突变的DYKDDDDK-Rev-erbα (Ser55/59Ala)表达构建体转染，293/Rev-erbα (WT )细胞提取物在含（+）或不含（-）λ磷酸酶中孵育。 |
