| 货号 | 3221S |
| 反应种属 | Human/D.melanogaster |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-Ret (Tyr905) Antibody detects endogenous levels of Ret only when phosphorylated at tyrosine 905. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | The Ret proto-oncogene (c-Ret) is a receptor tyrosine kinase that functions as a multicompetent receptor complex in conjunction with other membrane-bound ligand-binding GDNF family receptors (1). Ligands that bind the Ret receptor include the glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin (2-4). Alterations in the corresponding Ret gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma and a congenital developmental disorder known as Hirschsprung’s disease (1,3). The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr905 dramatically inhibits Ret autophosphorylation activity (5). |
| 存放说明 | -20C |
| 计算分子量 | 175 |
Western blot analysis of recombinant human GST-Ret (His658-Asp1110) and extracts from the TT human medullary thyroid carcinoma cell line using Phospho-Ret (Tyr905) Antibody.用Phospho-Ret (Tyr905) Antibody对以下样品进行免疫印迹检测：重组的人的GST-Ret (His658-Asp1110)，TT human medullary thyroid carcinoma细胞系的提取物。 | |
Western blot analysis of recombinant human GST-Ret (His658-Asp1110), untreated or λ phosphatase-treated, using Phospho-Ret (Tyr905) Antibody (upper) and Ret Antibody #3220 (lower).用Phospho-Ret (Tyr905) Antibody (上) 和 Ret Antibody #3220 (下)对未处理的和经过λ 磷酸酶处理的重组的人的GST-Ret (His658-Asp1110)进行免疫印迹检测。 |
