Phospho-RCC1 (Ser11) Antibody
货号：
4210S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 4210S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-RCC1 (Ser11) Antibody recognizes endogenous levels of RCC1 protein only when phosphorylated at Ser11. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle and nuclear transport (1,2). Like other small GTPases, Ran is active in its GTP-bound form and inactive in its GDP-bound form. Nuclear RanGTP concentration is maintained through nuclear localization of guanine nucleotide exchange factor (GEF) activity, which catalyzes the exchange of bound GDP for GTP. RCC1 (regulator of chromatin condensation 1) is the only known RanGEF (3). RCC1 is dynamically chromatin-bound throughout the cell cycle, and this localization is required for mitosis to proceed normally (4,5). Appropriate association of RCC1 with chromatin is regulated through amino-terminal phosphorylation (5,6) and methylation (7). RCC1 regulation of RanGTP levels in response to histone modifications regulates nuclear import during apoptosis (8). In mitosis RCC1 is phosphorylated at Ser11, possibly by cyclinB/cdc2 (9-11). This phosphorylation may play a role in RCC1 interaction with chromatin and its RanGEF activity (6). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
参考图片Western blot analysis of extracts from HT-29 cells, untreated or synchronized in mitosis by thymidine block and released into nocodazole, using Phospho-RCC1 (Ser11) Antibody (upper) or RCC1 Antibody #3589 (lower). 使用Phospho-RCC1 (Ser11) Antibody (上图)或RCC1 Antibody #3589 (下图)，免疫印迹（Western Blot）分析HT-29细胞中Phospho-RCC1 (Ser11)和RCC1蛋白水平。细胞分为未处理组和在有丝分裂有协同作用的thymidine与nocodazole共同处理组。 |
