Phospho-Rb (Ser807/811) Antibody
货号：
9308S 基本售价：
3980.0 元 规格：
100ul
产品信息
概述| 货号 | 9308S |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 使用方法 | WB(1:1000)
IP (1:100) |
性能| 供应商 | CST |
| 背景 | The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).视网膜母细胞瘤肿瘤抑制蛋白Rb通过控制细胞周期的G1期限制点的进程来调控细胞增殖(1)。Rb具有3个功能特异的结合结构域并能够与转录因子家族的关键调节蛋白E2F、c-Abl酪氨酸激酶和具有保守的LXCXE模体蛋白相互作用(2-4)。CDK介导的细胞周期依赖性磷酸化能够抑制Rb靶向结合并允许细胞周期进程(5)。Rb的失活和随后的细胞周期进程似乎需要伴随周期蛋白E-CDK2磷酸化的cyclin D-CDK4/6初始磷酸化(6)。离体已经观察到不同CDK/cyclin复合物的特异性(6-8)，而且周期蛋白D1为第780位丝氨酸在体磷酸化所必需(9)。 |
| 存放说明 | -20C |
| 计算分子量 | 110 |
参考图片Western blot analysis of extracts from human fibroblasts synchronized by serum deprivation, using Phospho-Rb (Ser807/811) Antibody. Cells were synchronized for 24 hours then released by addition of serum and harvested at the times indicated. Cell cycle progression was verified by cyclin analysis and FACS. (Provided by John Boylan, Dupont/Merck, Delaware.)Western blot 检测血清剥夺同步化的人类成纤维细胞提取物，使用的抗体为Phospho-Rb (Ser807/811) Antibody。 细胞同步化24小时后加入血清以释放，在指定的时间收样。细胞周期进程由周期蛋白分析和流式细胞术确定。（本图由 John Boylan, Dupont/Merck, Delaware提供） |
