| 货号 | 9380S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-RanBP3 (Ser58) Antibody recognizes endogenous levels of RanBP3b protein only when phosphorylated at Ser58, and RanBP3a protein only when phosphorylated at corresponding residue Ser126. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | RanBP3 was originally identified as RanGTP binding protein located in the nucleus and involved in the nuclear exporting process (1). It functions as a cofactor for CRM1 nuclear export by binding to CRM1, stabilizing the RanGTP-CRM1-cargo interaction and promoting complex association with nuclear pore proteins (2,3). In the absence of Ran-bound GTP, RanBP3 prevents binding of CRM1 complex to the nuclear pore complex. In addition to CRM1, RanBP3 also has been shown to bind to RanGEF-RCC1 and increase the guanine nucleotide exchange activity of RCC1 for RanGTP-CRM1-Cargo (1,4). In some cases, as with β-catenin and Smad2/3, RanBP3 binding may mediate the target protein nuclear export in a Ran-dependent, but CRM1-independent manner (5,6). RanBP3 is phosphorylated at Ser58 through the PI3K/Akt or ERK/RSK pathway. This phosphorylation is important for RanBP3 function in nuclear export, likely due to stimulation of RCC1 activity (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
Western blot analysis of extracts from HeLa and COS-7 cells, serum-starved overnight and either left untreated or treated with hEGF #8916 (100 ng/ml), using Phospho-RanBP3 (Ser58) Antibody (upper) or RanBP3 Rabbit mAb (lower). 使用Phospho-RanBP3 (Ser58) Antibody (上图)或RanBP3 Rabbit mAb (下图)，免疫印迹（Western Blot）分析HeLa和COS-7细胞中Phospho-RanBP3 (Ser58)和RanBP3蛋白水平。细胞经血清饥饿过夜，随后进行未处理或hEGF #8916 (100 ng/ml)处理。 |
