Phospho-Rad18 (Ser403) Antibody
货号：
8393S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 8393S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000)
IP (1:50) |
性能| 供应商 | CST |
| 背景 | DNA damage, if not repaired, can lead to genome instability and tumorigenesis. Eukaryotic cells use multiple (sometimes overlapping) signaling pathways to respond to agents that cause various types of DNA lesions. Downstream molecules in DNA repair pathways converge on the sites of DNA damage, resulting in cell cycle arrest and repair or apoptosis (1). Rad18 is an E3 ubiquitin ligase recruited to sites of DNA damage. Along with the E2 ubiquitin ligase rad6, rad18 is responsible for monoubiquitination of DNA damage proteins including the replication clamp PCNA and the Fanconi anemia core protein FANCD2. Monoubiquitination of these proteins signals to downstream effector molecules and results in the repair of either post-replication repair lesions via the translesion synthesis (TLS) pathway or of DNA double strand breaks via homologous recombination (2-4). Phospho-proteomic studies indicate that serine 403 of rad18 may be phosphorylated by ATM/ATR in response to DNA damage-inducing agents (5,6).DNA损伤，如果不进行修复，能够导致基因组不稳定和肿瘤发生。真核细胞采用多个(有时会有重叠)信号通路应答引起各种类型DNA损伤的因素。DNA修复中的下游分子集中于DNA损伤位点，引起细胞周期捕获和修复或凋亡(1)。Rad18是一种可被招募至DNA损伤位点的E3泛素连接酶。连同E2泛素连接酶rad16，rad18负责DNA损伤蛋白(包括复制夹蛋白PCNA和Fanconi贫血核心蛋白FANCD2)的单泛素化。这些蛋白的单泛素化给下游的效应器分子发送信号并且通过跨损伤合成途径进行损伤后修复或者通过同源重组修复DNA双链断裂(2-4)。磷酸蛋白质组学研究表明rad18的第403位丝氨酸可以被ATM/ATR磷酸化以响应DNA损伤诱导物(5,6)。 |
| 存放说明 | -20C |
| 计算分子量 | 80, 90 |
参考图片Western blot analysis of extracts from 293 or HeLa cells, untreated or UV-treated, using Phospho-Rad18 (Ser403) Antibody (upper) or a total Rad18 antibody (lower).Western blot 方法检测293或Hela细胞，分为非处理组合紫外处理组，使用的抗体为Phospho-Rad18 (Ser403) Antibody (上图) 或 Rad18总蛋白抗体（下图）。 |
