Phospho-Progesterone Receptor (Ser345) Antibody
货号：
12783S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 12783S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Human progesterone receptor (PR) is expressed as two forms: the full length PR-B and the short form PR-A. PR-A lacks the first 164 amino acid residues of PR-B (1,2). Both PR-A and PR-B are ligand activated, but differ in their relative ability to activate target gene transcription (3,4). The activity of PR is regulated by phosphorylation; at least seven serine residues are phosphorylated in its amino-terminal domain. Three sites (Ser81, Ser102, and Ser162) are unique to full length PR-B, while other sites (Ser190, Ser294, Ser345, and Ser400) are shared by both isoforms (5). Phosphorylation of PR-B at Ser190 (equivalent to Ser26 of PR-A) is catalyzed by CDK2 (6). Mutation of Ser190 results in decreased activity of PR (7), suggesting that the phosphorylation at Ser190 may be critical to its biological function.人孕酮受体（PR）表现为两种形式：全长的孕酮受体B和短的孕酮受体A。PR A缺少PR B的第164个氨基酸残基（1,2）。PR A和PR B都是配体激活，但其激活靶基因转录的相对能力是不同的（3,4）。PR的活性受磷酸化调节。在其氨基末端区域，至少有七个丝氨酸残基被磷酸化。丝氨酸三个位点（81，102，162位）是全长PR特有的，而其他丝氨酸位点（190，294，345和400位）是两个亚型所共有的（5）。PR B丝氨酸（190位）（相当于PR A丝氨酸（26位））的磷酸化是由CDK2催化的(6)。丝氨酸（190位）的突变导致PR活性降低(7)，这表明丝氨酸（190位）的磷酸化对其生物功能来说是至关重要的。 |
| 存放说明 | -20C |
| 计算分子量 | 90 (PR-A), 118 (PR-B) |
参考图片Western blot analysis of extracts from T-47D cells grown for 48 hr in phenol red-free medium supplemented with 5% charcoal-stripped FBS, untreated (-) or promegestone (R5020)-treated (100 nM, 1 hr; +), using Phospho-Progesterone Receptor (Ser345) Antibody (upper) or Progesterone Receptor A/B (D8Q2J) XP® Rabbit mAb #8757 (lower).Western blot方法检测T-47D细胞提取物，细胞在不含酚红，含5%活性炭/葡聚糖处理的FBS中培养48小时，之后细胞不处理（-）或普美孕酮(R5020)处理（100 nM, 1 hr; +），使用的抗体为Phospho-Progesterone Receptor (Ser345) Antibody (上图) 或Progesterone Receptor A/B (D8Q2J) XP® Rabbit mAb #8757 (下图). |
