| 货号 | 2640S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-PRAS40 (Thr246) Antibody detects endogenous levels of PRAS40 protein only when phosphorylated at Thr246. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 protein (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with Raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4). mTORC1 in turn phosphorylates PRAS40 at Ser183 (5). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
Western blot analysis of extracts from NIH/3T3 cells, serum-starved for 14 hours and then either left untreated or treated with insulin (150 nM) for 15 minutes, using Phospho-PRAS40 (Thr246) Antibody. Western blot 方法检测细胞提取物：血清饥饿14小时的NIH/3T3细胞，然后未处理和insulin (150 nM)处理15分钟，使用的抗体是Phospho-PRAS40 (Thr246)Antibody。 | |
Western blot analysis of extracts from NIH/3T3 cells, serum-starved for 14 hours and then either left untreated or treated with insulin (150 nM) for 15 minutes, using Phospho-PRAS40 (Thr246) Antibody. The experiment was performed in the presence of phospho-peptide specific to phospho-PRAS40 (Thr246) (upper panel), the corresponding nonphospho-peptide (middle panel) and in the absence of any blocking peptide (lower panel). Western blot 方法检测细胞提取物：血清饥饿14小时的NIH/3T3细胞，然后未处理和insulin (150 nM)处理15分钟，使用的抗体是Phospho-PRAS40 (Thr246)Antibody。上图是使用特异性识别phospho-PRAS40 (Thr246)的磷酸化肽段，中图是对应的非磷酸化肽段，下图是不用任何封闭肽。 |
