| 货号 | 5936S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-PRAS40 (Ser183) Antibody recognizes endogenous levels of PRAS40 protein only when phosphorylated at Ser183. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 protein (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with Raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4). mTORC1 in turn phosphorylates PRAS40 at Ser183 (5). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
Western blot analysis of extracts from HeLa cells, untreated or treated with LY294002 #9901 (with and without serum starvation) or with Rapamycin #9904, using Phospho-PRAS40 (Ser183) Antibody (upper) or PRAS40 (D23C7) XP® Rabbit mAb #2691 (lower). Weatern blot 分析Hela细胞提取物，未处理组和LY294002 #9901处理组（血清饥饿或未饥饿）及Rapamycin #9904处理组，所用抗体为Phospho-PRAS40 (Ser183) Antibody (上) 或 PRAS40 (D23C7) XP™ Rabbit mAb兔单抗 #2691 (下)。 | |
Western blot analysis of extracts from HeLa cells treated with hIGF-I #8917, in the absence (-) or presence (+) of λ phosphatase, using Phospho-PRAS40 (Ser183) Antibody (upper) or PRAS40 (D23C7) XP® Rabbit mAb #2691 (lower). Weatern blot 分析采用hIGF-I #8917处理的 Hela细胞提取物， λ 磷酸酶处理（+）或未处理（—），所用抗体为Phospho-PRAS40 (Ser183) Antibody (上) 或 PRAS40 (D23C7) XP™ Rabbit mAb兔单抗 #2691 (下)。 |
