| 货号 | 3804S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-PPIG (Ser376) Antibody detects endogenous levels of PPIG protein only when phosphorylated at Ser376. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | PPIG belongs to a highly conserved class of cyclophilins that function as peptidyl-prolyl-isomerases (PPIases) to catalyze the conversion of cis-proline to trans-proline in a polypeptide chain (1-4). PPIG contains an amino-terminal cyclophilin domain followed by Nopp140 repeats that are involved in its function as a nuclear chaperone (5). The carboxy-terminal of PPIG contains a SR (arginine-serine dipeptide repeat) domain (3,4) that is involved in pre-mRNA splicing and processing (6). PPIG interacts with the carboxy-terminal domain of RNA polymerase II as well as several other SR family splicing factors. These interactions lead to changes in localization and conformation and suggest a regulatory role in transcription and pre-mRNA splicing in the elongating RNA polymerase complex (7,8). PPIG is found in the nuclear matrix and nuclear speckles and is involved in the regulation of gene expression. PPIG shows a predominantly diffuse cytoplasmic distribution at the onset of mitosis, and in late telophase the isomerase is recruited to the newly formed nuclei (9). |
| 存放说明 | -20C |
| 计算分子量 | 110 |
Western blot analysis of extracts from various cell lines using Phospho-PPIG (Ser376) Antibody. 使用Phospho-PPIG (Ser376) Antibody，免疫印迹（Western Blot）分析在不同细胞系中Phospho-PPIG (Ser376)蛋白水平。 | |
Western blot analysis of extracts from RL-7 and HEK-293 cells, using Phospho-PPIG (Ser376) Antibody alone (A) or preincubated with either phospho-PPIG (Ser376) peptide (B) or nonphospho-PPIG (Ser376) peptide (C). 使用Phospho-PPIG (Ser376) Antibody，免疫印迹（Western Blot）分析在不同细胞系中Phospho-PPIG (Ser376)蛋白水平，其中A图仅使用抗体，B图之前孵育phospho-PPIG (Ser376) peptide，C图之前孵育nonphospho-PPIG (Ser376) peptide。 |
