| 货号 | 4510S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-PLCγ1 (Ser1248) Antibody detects PLCγ1 only when phosphorylated at Ser1248. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ and PLCε. The PLCβ subfamily includes four members, PLCβ1-4. All four members of the subfamily are activated by α- or β-γ-subunits of the heterotrimeric G-proteins (2,3). Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of Ser1105 by PKA or PKC inhibits PLCβ3 activity (4,5). Ser537 of PLCβ3 is phosphorylated by CaMKII, and this phosphorylation may contribute to the basal activity of PLCβ3. PLCγ is activated by both receptor and nonreceptor tyrosine kinases (6). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783 and 1245 (7). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (8). |
| 存放说明 | -20C |
| 计算分子量 | 155 |
Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated, using Phospho-PLCγ1 (Ser1248) Antibody (upper) or PLCγ1 Antibody #2822 (lower). 对未处理或用PDGF刺激的NIH/3T3细胞抽提液物，使用Phospho-PLCγ1 (Ser1248)抗体（上图）或PLCγ1抗体#2822（下图）进行Western blot分析。 | |
Western blot analysis of extracts from Jurkat or COS cells, untreated or TPA/UV treated, using Phospho-PLCγ1 (Ser1248) Antibody. 对Jurkat或COS细胞，未处理或TPA/UV处理，使用Phospho-PLCγ1 (Ser1248)抗体进行Western blot分析。 |
