Phospho-PLCbeta3 (Ser1105) Antibody
货号：
2484S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 2484S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-PLCbeta3 (Ser1105) Antibody detects endogenous levels of PLCbeta3 only when phosphorylated at serine 1105. The antibody does not cross-react with phosphorylated PLCbeta1, PLCbeta2, PLCbeta4 or other PLCs. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ and PLCε. The PLCβ subfamily includes four members, PLCβ1-4. All four members of the subfamily are activated by α- or β-γ-subunits of the heterotrimeric G-proteins (2,3).
Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of Ser1105 by PKA or PKC inhibits PLCβ3 activity (4,5). Ser537 of PLCβ3 is phosphorylated by CaMKII, and this phosphorylation may contribute to the basal activity of PLCβ3. PLCγ is activated by both receptor and nonreceptor tyrosine kinases (6).
PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783 and 1245 (7). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (8). |
| 存放说明 | -20C |
| 计算分子量 | 150 |
参考图片Western blot analysis of extracts from HeLa cells, untreated or PMA-treated for the indicated times, using Phospho-PLCbeta3 (Ser1105) Antibody (upper) or PLCbeta3 Antibody #2482 (lower). 对HeLa细胞，未处理或PMA处理适当时间，使用Phospho-PLCbeta3 (Ser1105)抗体（上图）或PLCbeta3抗体#2482（下图）进行Western blot分析。 |
