Phospho-PKA C (Thr197) Antibody
货号：
4781S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 4781S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-PKA C (Thr197) Antibody detects endogenous levels of PKA C (-alpha, -beta and -gamma) only when phosphorylated at Thr197. This antibody does not cross-react with PKA C phosphorylated at other sites. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The second messenger cyclic AMP (cAMP) activates cAMP-dependent protein kinase (PKA or cAPK) in mammalian cells and controls many cellular mechanisms such as gene transcription, ion transport, and protein phosphorylation (1). Inactive PKA is a heterotetramer composed of a regulatory subunit (R) dimer and a catalytic subunit (C) dimer. In this inactive state, the pseudosubstrate sequences on the R subunits block the active sites on the C subunits. Three C subunit isoforms (C-α, C-β, and C-γ) and two families of regulatory subunits (RI and RII) with distinct cAMP binding properties have been identified. The two R families exist in two isoforms, α and β (RI-α, RI-β, RII-α, and RII-β). Upon binding of cAMP to the R subunits, the autoinhibitory contact is eased and active monomeric C subunits are released. PKA shares substrate specificity with Akt (PKB) and PKC, which are characterized by an arginine at position -3 relative to the phosphorylated serine or threonine residue (2). Substrates that present this consensus sequence and have been shown to be phosphorylated by PKA are Bad (Ser155), CREB (Ser133), and GSK-3 (GSK-3α Ser21 and GSK-3β Ser9) (3-5). In addition, combined knock-down of PKA C-α and -β blocks cAMP-mediated phosphorylation of Raf (Ser43 and Ser259) (6). Autophosphorylation and phosphorylation by PDK-1 are two known mechanisms responsible for phosphorylation of the C subunit at Thr197 (7). |
| 存放说明 | -20C |
| 计算分子量 | 42 |
参考图片Western blot analysis of extracts from C6 and NIH/3T3 cells, untreated or treated with lambda phosphatase (PPase), using Phospho-PKA C (Thr197) Antibody (upper) or PKA C-alpha Antibody #4782 (lower). Western blot方法检测C6和NIH/3T3细胞提取物,细胞未处理或用lambda磷酸酶（PPase）处理，使用的抗体为Phospho-PKA C (Thr197) Antibody (上图) 或PKA C-alpha Antibody #4782 (下图)。 |
