| 货号 | 3061S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-PDK1 (Ser241) Antibody detects PDK1 only when phosphorylated at Ser241. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways (1,2) including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK (3). Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis.Several serine sites (Ser25, Ser241, Ser393/396 and Ser410) are phosphorylated on PDK1 in unstimulated human embryo kidney 293 cells, as well as IGF-1 stimulated cells (4). Phosphorylation on the activation loop Ser241 by autophosphorylation is necessary for PDK1 activity (4). |
| 存放说明 | -20C |
| 计算分子量 | 58 to 68 |
Western blot analysis of extracts from NIH/3T3 cells (starved for 16 hours) treated with PDGF (50 ng/ml), using Phospho-PDK1 (Ser241) Antibody (upper) or control PDK1 antibody (lower). The phospho-specificity of the antibody was confirmed by treating the membrane with calf intestinal alkaline phosphatase (CIP) after Western transfer.Western blot 检测分析用 PDGF (50 ng/ml)处理的饥饿16小时的NIH/3T3细胞提取物，使用的抗体是 Phospho-PDK1 (Ser241) Antibody（上图）和control PDK1 antibody（下图）。Western转膜后，用小牛肠碱性磷酸酶 (CIP) 处理膜来确认抗体识别磷酸化蛋白的特异性。 | |
Western blot analysis of extracts from 293 cells transiently transfected with DNA constructs expressing Wild-type PDK1 or S241A mutant PDK1, treated with IGF-1, using Phospho-PDK1 (Ser241) Antibody (upper) or control PDK1 Antibody #3062 (lower). (Cell lysates provided by Dr. M. Scheid, Ontario Cancer Institute, University Health Network, Toronto.)Western blot 检测分析293细胞提取物，用表达野生型PDK1和S241A突变的PDK1的DNA载体瞬转后，用IGF-1处理；使用的抗体是 Phospho-PDK1 (Ser241) Antibody （上图）和control PDK1 Antibody #3062（下图）。细胞裂解物由Dr. M. Scheid, Ontario Cancer Institute, University Health Network, Toronto友情赠送。 |
