| 货号 | 2541S |
| 反应种属 | Human/Mouse/Rat/Monkey/Pig |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Phospho-Paxillin (Tyr118) Antibody detects endogenous levels of paxillin only when phosphorylated at tyrosine 118. The antibody does not cross-react with other tyrosine phosphorylated proteins. |
| 使用方法 | WB(1:1000) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Paxillin is a multi-domain protein that localizes primarily to focal adhesion sites to the extracellular matrix (1). Paxillin is one of the key components of integrin signaling and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by another focal adhesion component, focal adhesion kinase (FAK), at Tyr118 (3,4). Phospho-Tyr118 may provide a docking site for recruitment of other signaling molecules to focal adhesions. It has been shown that the SH2 domain of Crk binds to the phosphorylated Tyr118 of paxillin (5). |
| 存放说明 | -20C |
| 计算分子量 | 68 |
Confocal immunofluorescent analysis of Hela cells -/+ lamda phosphatse labeled with Phospho-Paxillin (Tyr118) Antibody (green). Note the robust signal in the untreated cells (left) versus the lambda phosphatse treated cells (right).Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). Red = phalloidin555.使用Phospho-Paxillin (Tyr118) 抗体（绿色）对-/+ lamda磷酸酶标记的HeLa细胞进行激光共聚焦免疫荧光分析。注意相对lambda磷酸酶处理（右）细胞未处理细胞（左）中强烈的信号。蓝色假色=DRAQ5™（DNA荧光燃料）。红色=鬼笔环肽555. | |
Western blot analysis of untreated and lambda-phosphatase treated COS cells, using Phospho-Paxillin (Tyr118) Antibody (left) and Paxillin Antibody #2542 (right).使用Phospho-Paxillin (Tyr118) 抗体（左）和Paxillin抗体#2542（右）对未处理或经过lambda磷酸酶处理的COS细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from various cell lines using Phospho-Paxillin (Tyr118) Antibody.使用Phospho-Paxillin (Tyr118) 抗体对多种细胞提取物进行western blot分析。 |
