| 货号 | 2329S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | PAR-4 (prostate apoptosis response-4) was identified as a protein that is upregulated in prostate tumor cells undergoing apoptosis (1). Additionally, in parallel studies PAR-4 was found in the yeast two-hybrid system to bind to the Wilms tumor suppressor protein WT1 and may modulate WT1-medated transcriptional activation (2). PAR-4 contains a leucine zipper domain and a death domain and has been implicated as an effector of apoptosis during tumorigenesis as well as in neurodegenerative disorders (3,4). PAR-4 is widely expressed in normal tissues but can be downregulated in some tumor types. The mechanism of PAR-4 mediated apoptosis regulation appears to be complex and dependent on the cellular context. Studies have indicated roles for PAR-4 in activation of the Fas-FADD-caspase-8 pathway as well as inhibition of the NF-κB pro-survival pathway (5-7). Its activity is likely to depend on the cellular context and post-translational modifications. For instance, phosphorylation of PAR-4 by Akt prevents its nuclear translocation thereby promoting cell surivival (8). In contrast, phoshorylation of rat PAR-4 at T155 by PKA appears to positively regulate its apoptotic activity (9). |
| 存放说明 | -20C |
| 计算分子量 | 43 |
Western blot analysis of extracts from HeLa cells, untreated or forskolin-treated (30 μM), using Phospho-PAR-4 (Thr163) Antibody (upper) or total PAR-4 Antibody #2328 (lower). Western blot 检测HeLa细胞提取物，未处理或forskolin (30 μM)处理，使用抗体为Phospho-PAR-4 (Thr163) Antibody （上图）或 total PAR-4 Antibody #2328 （下图）。 | |
Western blot analysis of extracts from HeLa cells, untreated or treated with Calyculin A (100 nM, 5 minutes), using Phospho-PAR-4 (Thr163) Antibody (upper) or total PAR-4 Antibody #2328 (lower). Western blot 检测HeLa细胞提取物，未处理或Calyculin A (100 nM, 5 min)处理，使用抗体为Phospho-PAR-4 (Thr163) Antibody （上图）或 total PAR-4 Antibody #2328 （下图）。 |
