| 货号 | 3541S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:200) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication and molecular chaperoning activities (1,2). The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase (3,4).核磷蛋白(NPM；又称B23、Numatrin或N038)是一种高丰度的核仁磷酸化蛋白。它拥有几个独特的细胞职能，包括核糖体组装和运输，胞质/胞核运输，DNA聚合酶α活性的调节，中心体复制和分子伴侣活性(1,2)。NPM的编码基因也因为和间变性淋巴瘤激酶(ALK)酪氨酸受体激酶的融合而广为人知。NPM部分通过提供一个二聚体结构域促进转化，最终激活融合激酶(3,4)。 |
| 存放说明 | -20C |
| 计算分子量 | 38 |
Western blot analysis of extracts from HeLa cells synchronized at various stages of the cell cycle, using Phospho-NPM (Thr199) Antibody (upper) or NPM Antibody #3542 (lower).western blot方法检测细胞周期不同阶段同步化的HeLa细胞提取物，使用的抗体为 Phospho-NPM (Thr199) Antibody (上图) 或NPM Antibody #3542 (下图). | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using Phospho-NPM (Thr199) Antibody.免疫组织化学方法检测石蜡包埋人类乳腺癌组织，显示核定位，使用的抗体为Phospho-NPM(Thr199) Antibody。 | |
Confocal immunofluorescent analyis of HT-29 cells, untreated (left) or λ-phosphatase-treated (right), using Phospho-NPM (Thr199) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光方法检测HT-29 细胞， 未处理 (左图) 和λ 磷酸酶处理(右图), 检测抗体为Phospho-NPM (Thr199) Antibody ，呈绿色。肌动蛋白纤维由DY-554 鬼笔环肽标记，呈红色。蓝色伪彩为DNA荧光染料（产品信息为 DRAQ5®#4084 ）。 |
