| 货号 | 4206S |
| 反应种属 | Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-NMDAR2A (Tyr1246) Antibody detects endogenous levels of NMDAR2A only when phosphorylated at Tyr1246. The antibody may also detect NMDAR2B when phosphorylated at the conserved Tyr1252. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | N-methyl-D-aspartate receptor (NMDAR) forms a heterodimer of at least one NR1 and one NR2A-D subunit. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits. NR1 subunits bind the co-agonist glycine and NR2 subunits bind the neurotransmitter glutamate. Activation of the NMDA receptor or opening of the ion channel allows flow of Na+ and Ca2+ ions into the cell, and K+ out of the cell (1). Each subunit has a cytoplasmic domain that can be directly modified by the protein kinase/phosphatase (2). PKC can phosphorylate the NR1 subunit (NMDAR1) of the receptor at Ser890/Ser896, and PKA can phosphorylate NR1 at Ser897 (3). The phosphorylation of NR1 by PKC decreases its affinity for calmodulin, thus preventing the inhibitory effect of calmodulin on NMDAR (4). The phosphorylation of NR1 by PKA probably counteracts the inhibitory effect of calcineurin on the receptor (5). NMDAR mediates long-term potentiation and slow postsynaptic excitation, which play central roles in learning, neurodevelopment, and neuroplasticity (6).EphrinB2 binding to the receptor EphB leads to the activation of Src family tyrosine kinases, which phosphorylate NMDAR2B at Tyr1252, Tyr1336 and Tyr1472. In turn, phosphorylated NMDAR2B enhances the ability of the functional NMDA receptor to regulate Ca2+ influx in response to glutamate (7). The phosphorylation site of NMDAR2A at Tyr1246 is the conserved site of NMDAR2B at Tyr1252 and was independently identified at Cell Signaling Technology (CST) using PhosphoScan®, CSTs MS/MS platform for phosphorylation site discovery. Phosphorylation of NMDAR2A at Tyr1246 was observed in extracts isolated from ischemic rat brain. For additional information please visit PhosphoSitePlus®, CSTs modification site knowledgebase, at www.phosphosite.org. |
| 存放说明 | -20C |
| 计算分子量 | 180 |
Western blot analysis of extracts from rat brain, either sham-operated or 15 min ischemia, using Phospho-NMDAR2A (Tyr1246) Antibody (upper) or NMDAR2A Antibody (lower). Western blot分析大鼠大脑提取物，假手术组或15分钟缺血，使用的抗体是Phospho-NMDAR2A (Tyr1246) Antibody 兔多抗（上图）或NMDAR2A Antibody 兔多抗（下图）。 |
