| 货号 | 5026S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Myosin IIa (Ser1943) Antibody detects endogenous levels of myosin IIa protein only when phosphorylated at Ser1943. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7). |
| 存放说明 | -20C |
| 计算分子量 | 230 |
Western blot analysis of extracts from HeLa, A-431 and 293T cells, untreated or treated with λ phosphatase and calf intestinal phosphatase (CIP), using Phospho-Myosin IIa (Ser1943) Antibody (upper) or Myosin IIa Antibody #3403 (lower). 用Phospho-Myosin IIa (Ser1943) Antibody (上图)或Myosin IIa Antibody #3403 (下图)，免疫印迹（Western Blot）分析HeLa、A-431和293T细胞中Phospho-Myosin IIa和Myosin IIa蛋白水平。细胞分为λ phosphatase处理组和calf intestinal phosphatase (CIP)处理组。 | |
Western blot analysis of extracts from A-431 cells, untreated or treated with λ phosphatase and calf intestinal phosphatase (CIP), using Phospho-Myosin IIa (Ser1943) Antibody. The antibody was pre-incubated with peptides corresponding to unphosphorylated Myosin IIa or Myosin IIa phosphorylated at Ser1943, as indicated. 使用Phospho-Myosin IIa (Ser1943) Antibody，免疫印迹（Western Blot）分析A-431细胞中Phospho-Myosin IIa(Ser1943)蛋白水平。细胞分为λ phosphatase处理组和calf intestinal phosphatase (CIP)处理组。该抗体与相应的多肽预先孵育，分别是未磷酸化的Myosin IIa多肽和在Ser1943位点磷酸化的Myosin IIa多肽，如图所示。 |
