| 货号 | 9591S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | MSK1, a mitogen and stress activated protein kinase, is activated by Erk as well as p38 MAPK in response to growth factors and cellular stress, respectively (1). MSK1 resembles RSK because it has two kinase domains connected by a regulatory linker region (2). Phosphorylation of RSK1 at Ser364 and Ser381 is critical for RSK1 activity (3). These sites are analogous to Ser360 and Ser376 of MSK1, which may be important for MSK1 activity as well. |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Western blot analysis of extracts from RSK1, RSK2, RSK3 or MSK1 transfected 293 cells, untreated or TPA-treated (200 nM), using Phospho-MSK1 (Ser376) Antibody.Western blot方法检测细胞提取物：转染RSK1、 RSK2、 RSK3 、MSK1，未处理和TPA (200 nM)处理的293细胞。使用的抗体是Phospho-MSK1 (Ser376) Antibody。 | |
Western blot analysis of extracts from 293 cells, untreated, TPA-treated or UV-treated, using Phospho-MSK1 (Ser376) Antibody.Western blot方法检测细胞提取物：未处理、TPA (200 nM)处理和紫外照射的293细胞。使用的抗体是Phospho-MSK1 (Ser360) Antibody。 | |
Western blot analysis of extracts from 293 cells transfected with MSK1 or various mutants of MSK1, then treated with TPA (200 nM), and analyzed using Phospho-MSK1 (Ser376) Antibody.Western blot方法检测细胞提取物：转染MSK1、各种MSK1突变体，TPA (200 nM)处理的293细胞。使用的抗体是Phospho-MSK1 (Ser376) Antibody。 |
