Phospho-Mnk1 (Thr197/202) Antibody
货号：
2111S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 2111S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Mnk1 (Thr197/202) Antibody detects endogenous levels of Mnk1 only when phosphorylated at threonines 197 and 202. The antibody also cross-reacts with phosphorylated Mnk2a and Mnk2b. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148, and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin. |
| 存放说明 | -20C |
| 计算分子量 | 50 |
参考图片Western blot analysis of extracts from NIH/3T3 cells using Phospho-Mnk1 (Thr197/202) Antibody (upper) and Mnk1 (C4C1) Rabbit mAb #2195 (lower). The cells were starved for 24 hours in serum-free medium and then either untreated (-) or treated with serum for 30 minutes (+). 使用Phospho-Mnk1 (Thr197/202) Antibody (上图)和Mnk1 (C4C1) Rabbit mAb兔单抗 #2195 (下图)，免疫印迹（Western Blot）分析在NIH/3T3细胞系中Phospho-Mnk1 (Thr197/202)和Mnk1 (C4C1)蛋白水平。细胞经无血清饥饿培养24小时，然后分两组分别为不加血清(-)和加血清处理30分钟(+)。 |
