| 货号 | 3121S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-Met (Tyr1349) Antibody detects endogenous levels of Met only when phosphorylated at tyrosine 1349. This antibody may cross-react with activated EGF, PDGF, insulin and FGF receptors. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, Met is an attractive cancer therapeutic and diagnostic target (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 145 |
Western blot analysis of extracts from mIMCD-3 cells, untreated or HGF-stimulated (40 ng/ml for 2 minutes), using Phospho-Met (Tyr1349) Antibody (upper) or Met antibody (lower).用抗体Phospho-Met (Tyr1349) Antibody (上)或 Met antibody (下)对未处理的或HGF (40 ng/ml ，2 minutes)刺激过的mIMCD-3细胞的提取物进行免疫印迹检测。 | |
Western blot analysis of extracts from Vero cells, treated with In1B (3 nM) for the indicated times, using Phospho-Met (Tyr1349) Antibody (upper) or Met antibody (lower). In1B is a c-Met activator (Shen, Y. et al. 2000, Cell 103, 501-510). (In1B provided by Dr. K. Ireton, University of Toronto, Canada.)用抗体Phospho-Met (Tyr1349) Antibody (上)或 Met antibody (下)对In1B (3 nM)处理过的Vero细胞的提取物进行免疫印迹检测，细胞处理时间详见注释。In1B是 c-Met 激活剂 (Shen, Y. et al. 2000, Cell 103, 501-510)。 (In1B 由Dr. K. Ireton, University of Toronto, Canada提供)。 |
