| 货号 | 4836S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-MARK Family (Activation Loop) Antibody detects endogenous levels of phosphorylated MARK family members, MARK1 at threonine 215, MARK2 at threonine 208, and MARK3 at threonine 234 (A.K.A. 211 in isoforms 3-6) . This antibody does not react with MARK4. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Microtubule associated proteins regulate the stability of microtubules and control processes such as cell polarity/differentiation, neurite outgrowth, cell division and organelle trafficking (1). The MARK (MAP/microtubule affinity-regulating kinases) family (MARK1-4) of serine/threonine kinases was identified based on their ability to phosphorylate microtubule-associated proteins (MAPs) including tau, MAP2 and MAP4 (2-6). MARK proteins phosphorylate MAPs within their microtubule binding domains, causing dissociation of MAPs from microtubules and increased microtubule dynamics (2-4). In the case of tau, phosphorylation has been hypothesized to contribute to the formation of neurofibrillary tangles observed in Alzheimers disease. Overexpression of MARK leads to hyperphosphorylation of MAPs, morphological changes and cell death (4). The tumor suppressor kinase LKB1 phosphorylates MARK and the closely related AMP-kinases within their T-loops, leading to increased activity (7). |
| 存放说明 | -20C |
| 计算分子量 | 80 to 95 |
Western blot analysis of extracts from 293 cells transfected with either wild-type or threonine to alanine mutations at the respective phosphorylation sites of the MARK family members, using Phospho-MARK Family (Activation Loop) Antibody. Transfected constructs contain a GST and HA tag. Transfection efficiency monitored with HA Antibody, #2367. 使用Phospho-MARK Family (Activation Loop) Antibody，免疫印迹（Western Blot）分析293细胞Phospho-MARK蛋白水平，细胞分别转染wild-type或转染MARK蛋白家族磷酸化位点苏氨酸突变成丙氨酸。转染的质粒含有一个GST和HA标签。使用HA Antibody, #2367检测转染效率。 | |
Western blot analysis of extracts from Raji and BaF3 cells, showing endogenous levels of phosphorylated MARK family members, using Phospho-MARK Family (Activation Loop) Antibody. 使用Phospho-MARK Family (Activation Loop) Antibody，免疫印迹（Western Blot）分析Raji and BaF3细胞Phospho-MARK蛋白水平，结果显示内源性磷酸化MARK蛋白家族。 |
