Phospho-MAP2 (Thr1620/1623) Antibody
货号：
4544S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 4544S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-MAP2 (Thr1620/1623) Antibody detects transfected MAP2 (at a dilution of up to 1:20,000) only when phosphorylated at threonines 1620/1623. This antibody does not cross-react with phosphorylated tau. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. Serine 136, threonine 1620 and threonine 1623 of MAP2 are phosphorylated by GSK3 and p44/42 MAP kinases (2,3). Phosphorylation at threonine 1620/1623 by GSK3 inhibits MAP2 association with microtubules and microtubule stability (3). |
| 存放说明 | -20C |
| 计算分子量 | 82, 280 |
参考图片Western blot analysis of extracts from COS cells, untransfected (lane 1), or MAP2C transfected (lanes 2,3), using Phospho-MAP2 (Thr1620/1623) Antibody (upper) or MAP2 Antibody #4542 (lower). In lane 3, the lysate was treated with lamda phosphatase to demonstrate phospho-specificity of the antibody. (Human MAP2C construct is a generous gift from Dr. B. Shafit-Zagardo, Dept. of Pathology, Albert Einstein College of Medicine, Bronx, NY.) Western blot分析COS细胞，未转染（列1）或转染MAP2C（列2、3），使用的抗体是PPhospho-MAP2 (Thr1620/1623) Antibody 兔多抗（上图）或MAP2 Antibody 兔多抗#4542（下图）。列3中，裂解液经过λ磷酸酶处理，以显示该抗体的磷酸化特异性。（人HAP2C载体由Dept. of Pathology, Albert Einstein College of Medicine, Bronx, NY的Dr. B. Shafit-Zagardo赠送） |
