| 货号 | 4541S |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-F |
| 目标/特异性 | Phospho-MAP2 (Ser136) Antibody detects endogenous levels of all isoforms of MAP2 only when phosphorylated at serine 136. This antibody does not cross-react with phosphorylated tau. |
| 使用方法 | WB(1:1000) IF-F (1:100) |
| 供应商 | CST |
| 背景 | Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. Serine 136, threonine 1620 and threonine 1623 of MAP2 are phosphorylated by GSK3 and p44/42 MAP kinases (2,3). Phosphorylation at threonine 1620/1623 by GSK3 inhibits MAP2 association with microtubules and microtubule stability (3). |
| 存放说明 | -20C |
| 计算分子量 | 75, 82, 280 |
Western blot analysis of extracts from COS cells, untransfected (lane 1) or MAP2C-transfected (lanes 2,3), using Phospho-MAP2 (Ser136) Antibody (upper) or MAP2 Antibody #4542 (lower). In lane 3, the lysate was treated with lamda phosphatase to demonstrate phospho-specificity of the antibody. (Human MAP2C construct is a generous gift from Dr. B. Shafit-Zagardo, Dept. of Pathology, Albert Einstein College of Medicine, Bronx, NY.) Western blot分析COS细胞，未转染（列1）或转染MAP2C（列2、3），使用的抗体是Phospho-MAP2 (Ser136) Antibody 兔多抗（上图）或MAP2 Antibody 兔多抗 #4542（下图）。列3中，裂解液经过λ磷酸酶处理，以显示抗体的磷酸化特异性。（人HAP2C载体由 Dept. of Pathology, Albert Einstein College of Medicine, Bronx, NY的Dr. B. Shafit-Zagardo赠送） | |
Western blot analysis of extracts from PC12 cells, untreated or nocodazole-treated, using Phospho-MAP2 (Ser136) Antibody (upper) or MAP2 Antibody #4542 (lower). Western blot分析PC12细胞提取物，未处理或nocodazole处理，使用的抗体是Phospho-MAP2 (Ser136) Antibody 兔多抗（上图）或MAP2 Antibody 兔多抗 #4542（下图）。 | |
Confocal immunofluorescent analysis of rat brain, untreated (left) or λ-phosphatase treated (right), using Phospho-MAP2 (Ser136) Antibody (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析大鼠大脑，未经处理（左图）或经过λ磷酸酶处理（右图），使用的抗体是Phospho-MAP2 (Ser136) Antibody 兔多抗（绿色）和β3-Tubulin (TU-20) Mouse mAb 鼠单抗 #4466（红色）。蓝色伪彩=DRAQ5® #4084(荧光DNA染料)。 |
