| 货号 | 8176S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-LDHA (Tyr10) Antibody recognizes endogenous levels of LDHA protein only when phosphorylated at Tyr10. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD+, which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme. The LDHA promoter contains HIF-1α binding sites (1). LDHA expression is induced under hypoxic conditions (2). During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain. |
| 存放说明 | -20C |
| 计算分子量 | 37 |
Western blot analysis of extracts from various cell lines using Phospho-LDHA (Tyr10) Antibody. 对多个细胞系使用Phospho-LDHA (Tyr10)抗体进行Western blot分析。 | |
Western blot analysis of extracts from 293T cells transfected with a construct expressing DYKDDDDK-tagged wild-type (WT) full-length human LDHA (WT hLDHA-DDK) alone or in conjuction with full-length human FGFR1 (hFGFR1), using Phospho-LDHA (Tyr10) Antibody (upper) or DYKDDDDK Tag (9A3) Mouse mAb #8146 (lower). The DYKDDDDK-tagged WT hLDHA expression construct was kindly provided by Dr. Jing Chen at Emory University School of Medicine. 对单独转染DYKDDDDk-标签的野生型（WT）全长人LDHD（WT hLDHA-DDK）或联合全长人FGFR1（hFGFR1）的293T细胞使用Phospho-LDHA (Tyr10) Antibody （上图）或DYKDDDDK Tag (9A3) Mouse mAb 鼠单抗#8146（下图）进行Western blot分析。DYKDDDDK-tagged WT hLDHA表达载体由Dr. Jing Chen at Emory University School of Medicine提供。 |
