| 货号 | 2026S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Phospho-Lamin A/C (Ser22) Antibody detects endogenous levels of lamin A/C only when phosphorylated at Ser22. |
| 使用方法 | WB(1:1000) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 69, 78 |
Western blot analysis of extracts from HeLa and NIH/3T3 cells, hydroxyurea-treated (4 mM, 20 hours) to induce G1/S phase or paclitaxel-treated (100 nM, 20 hours) or nocodazole-treated (100 ng/ml, 20 hours) to induce G2/M phase, using Phospho-Lamin A/C (Ser22) Antibody (upper) or Lamin A/C Antibody #2032 (lower) as a loading control. 免疫印迹检测HeLa细胞和NIH/3T3细胞的细胞提取物，用hydroxyurea(羟基脲)(4 mM, 20 小时)处理诱导G1/S期，用paclitaxel（紫杉醇）(100 nM, 20 小时)或nocodazole（诺考达唑 (100 ng/ml, 20 h小时)处理诱导G2/M期：使用Phospho-Lamin A/C (Ser22) Antibody (上) 或 Lamin A/C Antibody #2032 (下) 作为上样对照。 | |
Western blot analysis of extract from G2/M phase HeLa cells untreated or treated with phosphatase, using Phospho-Lamin A/C (Ser22) Antibody (upper) or Lamin A/C Antibody (lower) as loading control. 免疫印迹分析G2/M期HeLa细胞未经过或经过磷酸酶处理的细胞提取物，使用Phospho-Lamin A/C (Ser22) Antibody (上) or Lamin A/C Antibody (下) 作为上样对照。 | |
Confocal immunofluorescent analysis of HeLa cells using Phospho-Lamin A/C (Ser22) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 免疫荧光实验分析HeLa细胞,使用Phospho-Lamin A/C (Ser22) Antibody (绿)。肌动蛋白微丝用DY-554鬼笔环肽（红）标记。Blue pseudocolor = DRAQ5™ (荧光DNA dye). |
