Phospho-IRS-1 (Ser789) Antibody
货号：
2389S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 2389S |
| 反应种属 | Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-IRS-1 (Ser789) Antibody detects transfected IRS-1 only when phosphorylated at serine 789. This antibody does not cross-react with related phospho-proteins. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10). |
| 存放说明 | -20C |
| 计算分子量 | 180 |
参考图片Western blot analysis of extracts from 293 cells overexpressing IRS-1, untreated or treated with insulin for 15 minutes, using Phospho-IRS-1 (Ser789) Antibody (upper) or IRS-1 Antibody #2382 (lower). The CIP treatment abolished the immunoreactivity of Phospho-IRS-1 (Ser789) Antibody with IRS-1 (upper, right). 对过表达IRS-1的293细胞（未处理或胰岛素处理15分钟），使用Phospho-IRS-1 (Ser789) Antibody（上图）或IRS-1 Antibody #2382（下图）进行Western blot分析。CIP处理消除了Phospho-IRS-1 (Ser789) Antibody与IRS-1（上右）的免疫反应性。 |
