Phospho-IRS-1 (Ser332/336) Antibody
货号：
2580S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 2580S |
| 反应种属 | Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-IRS-1 (Ser332/336) Antibody detects transfected levels of IRS-1 when phosphorylated at Ser332/336. It also detects IRS-1 protein when singly phosphorylated at Ser332 or Ser336 of human IRS-1. This antibody does not cross-react with other related phosphoproteins. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10). |
| 存放说明 | -20C |
| 计算分子量 | 180 |
参考图片Western blot analysis of cell extracts from CHO cells overexpressing insulin receptor and IRS-1, untreated or treated with insulin, using Phospho-IRS-1 (Ser332/336) Antibody (upper and middle) or IRS-1 Antibody #2382 (lower). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing. 对过表达胰岛素受体和IRS-1的CHO细胞（未处理或胰岛素刺激），使用Phospho-IRS-1 (Ser332/336) Antibody（上图和中图）或IRS-1 Antibody #2382（下图）进行Western blot分析。中间的印记在抗体探针加入前用小牛肠碱性磷酸酶（CIP）处理。 |
