Phospho-IRS-1 (Ser302) Antibody
货号：
2384S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 2384S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-IRS-1 (Ser 302) Antibody detects endogenous levels of IRS-1 only when phosphorylated at Ser302 of mouse IRS-1 or Ser307 of human IRS-1. This antibody does not detect IRS-1 phosphorylated at other sites. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10). |
| 存放说明 | -20C |
| 计算分子量 | 180 |
参考图片Western blot analysis of extracts from CHO IR/IRS-1 cells ( transfected with insulin receptor and IRS-1), untreated or insulin-treated (100 nM for 5 min), showing an increase in phospho-IRS-1 (Ser302) with insulin stimulation, using Phospho-IRS1 (Ser302) Antibody. To confirm phospho-specificity of the antibody, a duplicate membrane was treated with calf intestinal alkaline phosphatase (CIP) after Western transfer. 对CHO IR/IRS-1细胞（转染胰岛素受体和IRS-1）抽提液，未处理或100nM胰岛素处理5分钟，使用Phospho-IRS1 (Ser302) Antibody进行Western blot分析，显示胰岛素刺激后phospho-IRS-1 (Ser302)水平上升。抗体磷酸化特异性由western转膜后对副本膜进行小牛肠碱性磷酸酶（CIP）处理验证。 |
