| 货号 | 2385S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-IRS-1 (Ser1101) Antibody detects endogenous IRS-1 protein only when phosphorylated at serine 1101. The antibody cross-reacts with IRS-2. US Patent No. 7,731,964 and foreign equivalents assigned to Cell Signaling Technology, Inc. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10). |
| 存放说明 | -20C |
| 计算分子量 | 180 |
Western blot analysis of IRS-1 (#2382) antibody-immunoprecipitated pellets from differentiated C2C12 cells, unstimulated, insulin-stimulated (100 nM for 15 min) or PMA-stimulated (200 mM for 30 min), using Phospho-IRS-1 (Ser1101) Antibody (upper) or IRS-1 Antibody #2382 (lower). 对分化的C2C12细胞的IRS-1 (#2382)抗体免疫共沉淀颗粒，未处理或100nM胰岛素处理15分钟或200mM PMA处理30分钟，使用Phospho-IRS-1 (Ser1101) Antibody（上图）或IRS-1 Antibody #2382（下图）进行Western blot分析。 | |
Immunprecipitation of Phospho-IRS-1 from insulin treated MCF7 cell extracts using Phospho-IRS-1 (Ser1101) antibody (Lane 1). Lane 2: No antibody control. Lane 3: Input control. 对胰岛素处理的MCF7细胞裂解液使用Phospho-IRS-1 (Ser1101) antibody（列1）进行Phospho-IRS-1免疫共沉淀分析。列2：无抗体对照。列3：输入对照。 |
