| 货号 | 4126S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Phospho-HSL (Ser660) Antibody detects endogenous levels of HSL only when phosphorylated at Ser660 by PKA. This antibody does not cross-react with Ser563, Ser565 or Ser659 phosphorylated HSL. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | HSL (hormone-sensitive lipase) catalyzes the hydrolysis of triacylglycerol, the rate-limiting step in lipolysis. Lipolytic stimuli activate adenylyl cyclase and thus increase intracellular cAMP levels, which in turn activate protein kinase A (PKA). PKA phosphorylates HSL at Ser563, Ser659 and Ser660, which stimulates HSL activity (1,2). In contrast, AMPK phosphorylates HSL at Ser565, which reduces HSL phosphorylation at Ser563 by PKA and inhibits HSL activity (2,3). Recent work indicates that phosphorylation at Ser600 by p44/42 MAPKs also enhances the enzymatic activity of HSL (4). |
| 存放说明 | -20C |
| 计算分子量 | 81, 83 |
Western blot analysis of extracts from differentiated NIH/3T3-L1 cells treated with isoproterenol, using Phospho-HSL (Ser660) Antibody. western blot检测分化的NIH/3T3-L1细胞提取物，细胞用异丙肾上腺素处理，所用抗体为 Phospho-HSL (Ser660) Antibody。 | |
Confocal immunofluorescent analysis of 3T3-L1 adipocytes, isoproterenol-treated (left) or untreated (right), labeled with Phospho-HSL (Ser660) Antibody (red). Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦荧光免疫检测3T3-L1细胞，细胞未处理（左）或用磷酸酶处理（右），用Phospho-HSL (Ser660) Antibody（红）标记，显示在分化细胞中位于胞浆中。脂质滴用BODIPY® 493/503（绿）标记。蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5® #4084)。 |
