| 货号 | 2600S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Phospho-HP1γ (Ser83) Antibody detects endogenous levels of HP1γ protein only when phosphorylated on Ser83 (also referred to as Ser93 of the unprocessed form of HP1γ). This antibody does not cross-react with HP1α or HP1β proteins. |
| 使用方法 | WB(1:1000) IP (1:25) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure (1). All three HP1 family members (α, β and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome (2,3). HP1 proteins are approximately 25 kDa in size and each contains a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated on Lys9, a histone "mark" closely associated with centromeric heterochromatin (4,5). The variable hinge region binds both RNA and DNA in a sequence-independent manner (6). The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases and the p150 subunit of chromatin-assembly factor-1 (CAF1) (7-9). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells (10,11). HP1 proteins are subject to multiple types of post-translational modifications, including phosphorylation, acetylation, methylation, ubiquitination and sumoylation, suggesting multiple means of regulation (12-14). |
| 存放说明 | -20C |
| 计算分子量 | 22 |
Western blot analysis of whole cell extracts from HeLa cells, untreated (lanes 1 and 4), treated for 1 h with Forskolin (30 μM) and IBMX (0.5 mM) (lanes 2 and 3), or treated for 16 h with paclitaxel (500 nM) (lanes 5 and 6), using Phospho-HP1γ (Ser83) Antibody (upper panel) or HP1γ Antibody #2619 (lower panel). | |
使用Phospho-HP1γ (Ser83) Antibody (上图)或HP1γ Antibody #2619 (下图)，免疫印迹(Western blot)分析HeLa细胞中SirT6蛋白水平，分为未处理组 (第1和4道), Forskolin处理1个小时 (30 μM) and IBMX (0.5 mM) (第2和3道), 或者是 paclitaxel (500 nM) 处理16小时(第5和6道)。 |
