Phospho-HER2/ErbB2 (Tyr1248) Antibody
货号：
2247S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 2247S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-HER2/ErbB2 (Tyr1248) Antibody detects endogenous levels of ErbB2 only when phosphorylated at tyrosine 1248. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5). |
| 存放说明 | -20C |
| 计算分子量 | 185 |
参考图片Western blot analysis of activated recombinant HER2/ErbB2 and HER1/EGFR tyrosine kinase proteins, using Phospho-HER2/ErbB2 (Tyr1248) (upper), EGF Receptor (D38B1) XP® Rabbit mAb #4267 (middle) or HER2/ErbB2 (29D8) Rabbit mAb #2165 (lower). Phospho-HER2/ErbB2 (Tyr1248) specifically detects phosphorylated HER2/ErbB2 but not phosphorylated HER1/EGFR. Western blot法检测激活的重组HER2/ErbB2 和 HER1/EGFR酪氨酸激酶蛋白：使用抗体为：Phospho-HER2/ErbB2 (Tyr1248) (上)， EGF Receptor (D38B1) XP® Rabbit mAb #4267 (中)或 HER2/ErbB2 (29D8) Rabbit mAb #2165 (下)。抗体Phospho-HER2/ErbB2 (Tyr1248)特异检测磷酸化的HER2/ErbB2，但不能检测磷酸化的HER1/EGFR。 |
Western blot analysis of T47D and MCF7 cells untreated or treated with Neuregulin (100 ng/ml) for 5 minutes, using Phospho-HER2/ErbB2 (Tyr1248) Antibody (upper) or HER2/ErbB2 (D8F12) XP® Rabbit mAb #4290 (lower).Western blot法检测下列细胞提取物：未经处理的和用Neuregulin (100 ng/ml)刺激5分钟的T47D 和MCF7。使用抗体为：Phospho-HER2/ErbB2 (Tyr1248) (上)，或HER2/ErbB2 (D8F12) XP® Rabbit mAb #4290 (下)。 |
