Phospho-GSK-3β (Thr390) Antibody
货号：
3548S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 3548S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-GSK-3β (Thr390) Antibody detects endogenous levels of human GSK-3β protein only when phosphorylated at Thr390. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). Phosphorylation of Thr390 on GSK-3β was identified at Cell Signaling Technology (CST) using PhosphoScan®, CSTs LC-MS/MS platform for modification site discovery (6). The phosphorylation of this site was also reported by several other labs using phosphoproteomic analysis on mitotic cell extracts (7-10). |
| 存放说明 | -20C |
| 计算分子量 | 46 |
参考图片Western blot analysis of extracts from HeLa cells, either hydroxyurea-treated (4 mM, 20 hrs) to induce G1/S phase arrest or paclitaxel-treated (100 nM/ml, 20 hrs) for G2/M phase arrest, using Phospho-GSK-3β (Thr390) Antibody (upper) or GSK-3β (27C10) Rabbit mAb #9315 (lower) to show induction of phospho-GSK and equal loading. Western blot分析Hela细胞提取物，羟基脲(4 mM, 20 hrs)处理组诱导G1/S 期阻滞，紫杉醇处理组(100 nM/ml, 20 hrs)诱导G2/M阻滞，所用抗体为Phospho-GSK-3β (Thr390) Antibody (上) 和GSK-3β (27C10) Rabbit mAb 兔单抗#9315 (下) 用以显示phospho-GSK的诱导和相同上样量. |
