Phospho-GKAP (Ser346) Antibody
货号：
13638S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 13638S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-GKAP (Ser346) Antibody recognizes endogenous levels of GKAP protein only when phosphorylated at Ser346. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Guanylate kinase-associated protein (GKAP, DLGAP1 or SAPAP1) is part of the postsynaptic scaffolding complex that includes the PSD-95, SAP90, and SHANK proteins (1-3). GKAP links the synaptic protein SHANK to a PSD-95 complex that includes NMDA glutamate receptors (3,4). Synaptic activity induces ubiquitination of GKAP protein by the E3 ubiquitin ligase TRIM3, which results in decreased GKAP protein levels through degradation (5,6). GKAP protein turnover is regulated by a CaMKII-dependent, bidirectional mechanism. Synaptic over-excitation leads to CaMKIIα-mediated GKAP phosphorylation at Ser346, which induces polyubiquitination of GKAP and removal of the scaffold protein from synapses. In contrast, during low-level synaptic activity CaMKIIβ phosphorylates GKAP, which triggers dissociation of GKAP from the motor protein complex responsible for GKAP transport to the base of the synapse and its subsequent incorporation into the postsynaptic density (7). |
| 存放说明 | -20C |
| 计算分子量 | 90-150 |
参考图片Western blot analysis of extracts from mouse and rat brain using Phospho-GKAP (Ser346) Antibody. The phospho-specificity of Phospho-GKAP (Ser346) Antibody was verified by peptide blocking using a phosphopeptide or non-phosphopeptide encompassing residue Ser346. Western blot分析小鼠和大鼠大脑提取物，使用的抗体是Phospho-GKAP (Ser346) Antibody兔多抗。用一个靶向Ser346的磷酸化肽段或非磷酸化肽段进行肽段封闭以确认Phospho-GKAP (Ser346) Antibody的磷酸化特异性。 |
