| 货号 | 12745S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | The Grb-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ (8). Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2 (6,9). |
| 存放说明 | -20C |
| 计算分子量 | 110 |
Western blot analysis of cell extracts from serum-starved T-47D cells, untreated (-) or treated with neuregulin (100 ng/ml, 15 min; +), using Phospho-Gab1 (Tyr659) Antibody (upper) or Gab1 Antibody #3232 (lower). | |
Immunoprecipitation of phospho-Gab1 (Tyr659) from neuregulin-stimulated (100 ng/ml, 15 min) T-47D cell extracts using Normal Rabbit IgG #2729 (lane 2) or Phospho-Gab1 (Tyr659) Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Gab1 (Tyr659) Antibody. |
