| 货号 | 2486S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-FoxO1 (Ser319) Antibody detects exogenous levels of FoxO1 only when phosphorylated at serine 319. The antibody does not cross-reacts with FoxO1 phosphorylated at other sites nor with other phosphorylated family members. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9). |
| 存放说明 | -20C |
| 计算分子量 | 120 (GFP-FoxO1) |
Western blot analysis of extracts from untreated or serum-treated COS-7 cells exogenously expressing GFP-FoxO1, using Phospho-FoxO1 (Ser319) Antibody. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after Western transfer. Western blot方法检测分析未处理和血清处理的外源性表达GFP-FoxO1蛋白的COS-7细胞株提取物，使用的抗体是Phospho-FoxO1 (Ser319)。Western转膜后，分别用小牛肠碱性磷酸酶 (CIP) 处理和不处理的膜确认抗体识别磷酸化蛋白的特异性。 | |
Western blot analysis of extracts from serum-treated COS-7 cells untransfected, exogenously expressing FoxO4 or GFP-FoxO1, using Phospho-FoxO1 (Ser319) (left) or Phospho-FoxO1 (Ser319)/FoxO4 (Ser262) Antibody #2487 (right). Western blot方法检测分析血清处理的外源性表达FoxO4 和GFP-FoxO1蛋白的未转染处理的COS-7细胞株提取物，使用的抗体是Phospho-FoxO1 (Ser319)（左图）和Phospho-FoxO1 (Ser319)/FoxO4 (Ser262) Antibody #2487（右图）。 |
