| 货号 | 14170S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Forkhead box M1 (FoxM1) is a forkhead box family transcription factor that regulates a number of genes throughout the cell cycle to help control DNA replication, mitosis, and cell proliferation. FoxM1 expression increases during G1 and S and reaches maximum levels in G2/M (1-3). Nuclear translocation occurs just before entry into G2/M and is associated with FoxM1 phosphorylation (4). Phosphorylation of FoxM1 by MAPK (Ser331, Ser704), Cyclin/Cdk (Ser4, Ser35, Thr600, Thr611, Thr620, Thr627, Ser638), Plk1 (Ser715, Ser724), and Chk2 (Ser376) stabilizes and activates FoxM1 (4-8). Forkhead box M1 is expressed in all embryonic tissues but is restricted to proliferating tissues in adults (9). Recent studies have shown that FoxM1 expression is negatively regulated by p53 (10,11). Upregulation of FoxM1 is associated with many human cancers, including prostate, breast, lung, ovary, colon, pancreas, stomach, bladder, liver, and kidney, and may be associated with p53 mutations in some tumors (11,12). As a result, FoxM1 inhibitors have become a topic of interest for potential cancer therapy (13). |
| 存放说明 | -20C |
| 计算分子量 | 125 |
Western blot analysis of extracts from U-2 OS cells synchronized in mitosis with Nocodazole #2190 (100 ng/ml, 18 hr) and released into fresh medium for the indicated times, using Phospho-FoxM1 (Ser35) Antibody (upper), FoxM1 (D12D5) XP® Rabbit mAb #5436 (middle) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). | |
Western blot analysis of extracts from ACHN cells that are untreated (-) or synchronized in mitosis with Nocodazole #2190 (100 ng/ml, 18 hr; +), using Phospho-FoxM1 (Ser35) Antibody (upper), FoxM1 (D12D5) XP® Rabbit mAb #5436 (middle) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). | |
Western blot analysis of extracts from HeLa cells that are untreated (-), synchronized in S-phase by double thymidine block (2 mM, 18 hr; +), or synchronized in mitosis with double thymidine block followed by release into fresh medium containing Nocodazole #2190 (100 ng/ml, 18 hr; +), using Phospho-FoxM1 (Ser35) Antibody (upper), FoxM1 (D12D5) XP® Rabbit mAb #5436 (middle) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). |
