Phospho-Filamin A (Ser2152) Antibody
货号：
4761S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 4761S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Filamin A (Ser2152) Antibody detects endogenous levels of filamin A only when phosphorylated at serine 2152. This antibody also reacts with filamin C when it is phosphorylated at serine 2146. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Filamins are a family of dimeric actin binding proteins that function as structural components of cell adhesion sites. They also serve as a scaffold for subcellular targeting of signaling molecules (1). The actin binding domain (alpha-actinin domain) is located at the very amino-terminus, followed by as many as 24 tandem repeats of about 96 residues, and the dimerization domain is located at the carboxy-terminal end of th protein. In addition to actin filaments, filamins associate with other structural and signaling molecules such as beta-integrins, Rho/Rac/Cdc42, PKC, and insulin receptor, primarily through their carboxy-terminal half (1-3). Filamin A, the most abundant, and filamin B, less abundant, are widely expressed isoforms, while filamin C is predominantly expressed in muscle (1). Serine 2152 of filamin A is phosphorylated by PAK1, and this phosphorylation is required for PAK1-mediated actin cytoskeleton reorganization (4). |
| 存放说明 | -20C |
| 计算分子量 | 280 |
参考图片Western blot analysis of extracts from HEK293 and HeLa cells, untreated or lamda phosphatase-treated, using Phospho-Filamin A (Ser2152) Antibody (upper) and Filamin A Antibody #4762 (lower). 使用Phospho-Filamin A (Ser2152) Antibody (上图)和Filamin A Antibody #4762 (下图)，免疫印迹（Western Blot）分析HEK293和HeLa细胞中Phospho-Filamin A (Ser2152)和Filamin A蛋白水平，细胞分为未处理组和lamda phosphatase处理组。 |
