Phospho-FADD (Ser194) Antibody (Human Specific)
货号：
2781S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 2781S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-FADD (Ser194) Antibody (Human Specific) detects endogenous levels of human FADD protein only when phosphorylated at serine 194. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Fas-associated death domain (FADD or Mort 1) functions as an important adaptor in coupling death signaling from membrane receptors, such as the Fas ligand and TNF family (DR3, DR4 and DR5), to caspase-8 (1,2). FADD has a carboxy-terminal death domain, which interacts with the cytoplasmic tail of the membrane receptor, and an amino-terminal death effector domain, which interacts with caspase-8. Clustering of the receptors upon stimulation brings about FADD and caspase-8 oligomerization, activating the caspase signaling pathway. Human FADD is phosphorylated mainly at Ser194, while mouse FADD is phosphorylated at Ser191. In both cases, the phosphorylation is cell cycle-dependent (3) and may be related to its regulatory role in embryonic development and cell cycle progression (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 28 |
参考图片Western blot analysis of extracts from Jurkat and HeLa cells, hydroxyurea-treated (4 mM, 20 hours) or nocodazole-treated (1 µg/ml, 20 hours), using Phospho-FADD Ser194 Antibody (Human Specific) (upper) or control FADD Antibody (Human Specific) #2782 (lower). Western blot分析Jurkat和HeLa 细胞提取物，hydroxyurea（羟基脲） (4 mM, 20 hr)或（nocodazole）噻氨酯哒唑 (1 µg/ml, 20 hr)处理， 使用抗体为Phospho-FADD Ser194 Antibody (Human Specific) （上图）和control FADD Antibody (Human Specific) #2782（下图）。 |
