Phospho-Ephrin B (Tyr324/329) Antibody
货号：
3481S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 3481S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Ephrin B (Tyr324/329) Antibody detects transfected levels of ephrin B protein only when phosphorylated at tyrosines 324/329. The antibody cross-reacts with ephrins B1, B2 and B3 but does not cross-react with other tyrosine-phosphorylated proteins. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands: EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand; EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Eph receptors and ligands may be involved in many diseases including cancer (3). Both ephrin A and B ligands have dual functions. As RTK ligands, the ephrins stimulate the kinase activity of the Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5). Various stimuli can induce tyrosine phosphorylation of ephrin B, including binding to EphB receptors, activation of Src kinase, and stimulation by PDGF and FGF (6). Tyrosines 324/327 have been identified as major phosphorylation sites of ephrin B1 in vivo (7). |
| 存放说明 | -20C |
| 计算分子量 | 59 |
参考图片Western blot analysis of extracts from NIH/3T3 cells stably expressing ephrin B1, stimulated with its cognate receptor EphB2-Fc or with unfused Fc, using Phospho-Ephrin B (Tyr324/329) Antibody (upper) or ephrin B antibody (lower).用抗磷酸化Ephrin B(Tyr324/329)的抗体（上）或抗ephrin B的抗体（下）对经过EphB2-Fc或非融合的Fc刺激过的能稳定表达ephrin B1的NIH/3T3细胞的提取物进行免疫印迹检测。 |
